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Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the XRCC5 antibody detected the XRCC5 protein (arrow).
Confocal immunofluorescent analysis with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.

XRCC5 Antibody

CATALOG NUMBER: 56-143

Clonality:
Polyclonal
Tested Applications:
Flow, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This XRCC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-450 amino acids from the Central region of human XRCC5.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
83 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
XRCC5
Additional Names:
X-ray repair cross-complementing protein 5, 364-, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, Ku80, Ku86, Lupus Ku autoantigen protein p86, Nuclear factor IV, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), XRCC5, G22P2
Protein Accession Number:
P13010
Protein Gi Number:
125731
Ncbi Gene Id Number:
7520
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is the 80-kilodaltonsubunit of the Ku heterodimer protein which is also known asATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku isthe DNA-binding component of the DNA-dependent protein kinase, andit functions together with the DNA ligase IV-XRCC4 complex in therepair of DNA double-strand break by non-homologous end joining andthe completion of V(D)J recombination events. This genefunctionally complements Chinese hamster xrs-6, a mutant defectivein DNA double-strand break repair and in ability to undergo V(D)Jrecombination. A rare microsatellite polymorphism in this gene isassociated with cancer in patients of varying radiosensitivity.
Background Reference 1:
Gomes, B.C., et al. Oncol. Rep. 24(4):1079-1085(2010)
Background Reference 2:
Liu, Y., et al. Carcinogenesis 31(10):1762-1769(2010)
Background Reference 3:
Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press :
Background Reference 4:
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-143

List Size:
400 ul

List Price:

$519.00

Datasheet
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