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EDN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in ZR-75-1,U251,MDA-MB453,A549 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of EDN1 Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
EDN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

EDN1 Antibody

CATALOG NUMBER: 56-152

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EDN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-203 amino acids from the C-terminal region of human EDN1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:50~100
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EDN1
Additional Names:
Endothelin-1, Preproendothelin-1, PPET1, Endothelin-1, ET-1, Big endothelin-1, EDN1
Protein Accession Number:
P05305
Protein Gi Number:
119610
Ncbi Gene Id Number:
1906
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is proteolyticallyprocessed to release a secreted peptide termed endothelin 1. Thispeptide is a potent vasoconstrictor and is produced by vascularendothelial cells. Endothelin 1 also can affect the central nervoussystem. Two transcript variants encoding different isoforms havebeen found for this gene.
Background Reference 1:
Gonsalves, C., et al. J. Immunol. 185(10):6253-6264(2010)
Background Reference 2:
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Background Reference 3:
Feng, J., et al. Circulation 122 (11 SUPPL), S150-S155 (2010) :
Background Reference 4:
Nikopensius, T., et al. Birth Defects Res. PartClin. Mol. Teratol. 88(9):748-756(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-152

List Size:
400 ul

List Price:

$495.00

Datasheet
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