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Western blot analysis in A549 cell line lysates (35ug/lane).
ARRB1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of ARRB1 Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A549 cell line lysates (35ug/lane).

ARRB1 Antibody

CATALOG NUMBER: 56-170

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rabbit, Rat
Immunogen:
This ARRB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-363 amino acids from the C-terminal region of human ARRB1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARRB1
Additional Names:
Beta-arrestin-1, Arrestin beta-1, ARRB1, ARR1
Protein Accession Number:
P49407
Protein Gi Number:
20141238
Ncbi Gene Id Number:
408
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Members of arrestin/beta-arrestin protein family arethought to participate in agonist-mediated desensitization ofG-protein-coupled receptors and cause specific dampening ofcellular responses to stimuli such as hormones, neurotransmitters,or sensory signals. Arrestin beta 1 is a cytosolic protein andacts as a cofactor in the beta-adrenergic receptor kinase (BARK)mediated desensitization of beta-adrenergic receptors. Besides thecentral nervous system, it is expressed at high levels inperipheral blood leukocytes, and thus the BARK/beta-arrestin systemis believed to play a major role in regulating receptor-mediatedimmune functions. Alternatively spliced transcripts encodingdifferent isoforms of arrestin beta 1 have been described, however,their exact functions are not known.
Background Reference 1:
Shukla, A.K., et al. J. Biol. Chem. 285(39):30115-30125(2010)
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Lee, D.K., et al. Biochem. Biophys. Res. Commun. 395(2):185-189(2010)
Background Reference 4:
Kim, J.I., et al. Mol. Cancer Res. 8(4):569-577(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-170

List Size:
400 ul

List Price:

$495.00

Datasheet
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