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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
ACTN3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

ACTN3 Antibody

CATALOG NUMBER: 56-350

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ACTN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-437 amino acids from the Central region of human ACTN3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
103 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACTN3
Additional Names:
Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein, ACTN3
Protein Accession Number:
Q08043
Protein Gi Number:
215273967
Ncbi Gene Id Number:
89
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Alpha-actinin is an actin-binding protein with multipleroles in different cell types. This gene expression is limited toskeletal muscle. It is localized to the Z-disc and analogous densebodies, where it helps to anchor the myofibrillar actin filaments
Background Reference 1:
Rodriguez-Romo, G., et al. Eur. J. Appl. Physiol. 110(6):1099-1106(2010)
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Berman, Y., et al. Physiology (Bethesda) 25(4):250-259(2010)
Background Reference 4:
Ruiz, J.R., et al. ScandMed Sci Sports (2010) In press :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-350

List Size:
400 ul

List Price:

$495.00

Datasheet
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