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Western blot analysis of APEH antibody in A2058 cell line lysates (35ug/lane)
Western blot analysis of APEH antibody in A2058 cell line lysates (35ug/lane)

APEH Antibody

CATALOG NUMBER: 56-505

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This APEH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-204 amino acids from the N-terminal region of human APEH.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
81 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
APEH
Additional Names:
Acylamino-acid-releasing enzyme, AARE, Acyl-peptide hydrolase, APH, Acylaminoacyl-peptidase, Oxidized protein hydrolase, OPH, APEH, D3F15S2, D3S48E, DNF15S2
Protein Accession Number:
P13798
Protein Gi Number:
38258902
Ncbi Gene Id Number:
327
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
APEH is the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. APEH gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. The protein can play an important role in destroying oxidatively damaged proteins in living cells.
Background Reference 1:
Perrier,J., Giardina,T. Biol. Cell 94 (1), 45-54 (2002)
Background Reference 2:
Erlandsson,R., Boldog,F. Oncogene(7), 1293-1295 (1991)
Background Reference 3:
Erlandsson,R., Bergerheim,U.S. Oncogene(8), 1207-1211 (1990)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-505

List Size:
400 ul

List Price:

$495.00

Datasheet
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