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Western blot analysis of BCAT2 using rabbit polyclonal BCAT2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCAT2 gene.
BCAT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of BCAT2 using rabbit polyclonal BCAT2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCAT2 gene.

BCAT2 Antibody

CATALOG NUMBER: 56-721

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This BCAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-336 amino acids from the C-terminal region of human BCAT2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BCAT2
Additional Names:
Branched-chain-amino-acid aminotransferase, mitochondrial, BCAT(m), Placental protein 18, PP18, BCAT2, BCATM, BCT2, ECA40
Protein Accession Number:
O15382
Ncbi Gene Id Number:
587
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a branched chain aminotransferase foundin mitochondria. The encoded protein forms a dimer that catalyzesthe first step in the production of the branched chain amino acidsleucine, isoleucine, and valine. Multiple transcript variantsencoding different isoforms have been found for this gene.
Background Reference 1:
Coles, S.J., et al. Biochemistry 48(3):645-656(2009)
Background Reference 2:
Yennawar, N.H., et al. J. Biol. Chem. 281(51):39660-39671(2006)
Background Reference 3:
Goto, M., et al. J. Biol. Chem. 280(44):37246-37256(2005)
Background Reference 4:
Conway, M.E., et al. Biochemistry 43(23):7356-7364(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-721

List Size:
400 ul

List Price:

$495.00

Datasheet
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