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Western blot analysis of anti-Bcl-w BH3 domain Pab in HL-60 cell lysate. Bcl-w BH3 domain was detected using purified Pab.
Western blot analysis of Bcl-w3 using rabbit polyclonal Bcl-w BH3 Domain Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCL2L2 gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Bcl-w BH3 Domain Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the BCLW antibody detected the BCLW protein (arrow).
Western blot analysis of anti-Bcl-w BH3 domain Pab in HL-60 cell lysate. Bcl-w BH3 domain was detected using purified Pab.

Bcl-w Antibody

CATALOG NUMBER: 56-826

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-59 amino acids from human Bcl.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BCL2L2
Additional Names:
Bcl-2-like protein 2, Bcl2-L-2, Apoptosis regulator Bcl-W, BCL2L2, BCLW, KIAA0271
Protein Accession Number:
Q92843
Protein Gi Number:
296434404
Ncbi Gene Id Number:
599
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bcl-w is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis.
Background Reference 1:
Denisov, A.Y., et al., J. Biol. Chem. 278(23):21124-21128 (2003).
Background Reference 2:
Hinds, M.G., et al., EMBO J. 22(7):1497-1507 (2003).
Background Reference 3:
Middleton, G., et al., Development 128(3):447-457 (2001).
Background Reference 4:
Ross, A.J., et al., Nat. Genet. 18(3):251-256 (1998).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-826

List Size:
400 ul

List Price:

$495.00

Datasheet
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