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Western blot analysis in CEM cell line lysates (35ug/lane).
ACTG2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate cancinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in CEM cell line lysates (35ug/lane).

ACTG2 Antibody

CATALOG NUMBER: 57-103

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat
Immunogen:
This ACTG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343-371 amino acids from the C-terminal region of human ACTG2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACTG2
Additional Names:
Actin, gamma-enteric smooth muscle, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin, ACTG2, ACTA3, ACTL3, ACTSG
Protein Accession Number:
P63267
Protein Gi Number:
54036679
Ncbi Gene Id Number:
72
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Actins are highly conserved proteins that are involved invarious types of cell motility, and maintenance of thecytoskeleton. In vertebrates, three main groups of actin isoforms,alpha, beta and gamma have been identified. The alpha actins arefound in muscle tissues and are a major constituent of thecontractile apparatus. The beta and gamma actins co-exist in mostcell types as components of the cytoskeleton, and as mediators ofinternal cell motility. Actin, gamma 2, encoded by this gene, is asmooth muscle actin found in enteric tissues.
Background Reference 1:
Sun, Q., et al. J. Biol. Chem. 284(47):32582-32590(2009)
Background Reference 2:
Laasanen, J., et al. Fetal. Diagn. Ther. 23(1):36-40(2008)
Background Reference 3:
Goldberg, M.T., et al. J. Invest. Dermatol. 127(11):2645-2655(2007)
Background Reference 4:
Yonemoto, S., et al. Clin. Exp. Nephrol. 10(3):186-192(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-103

List Size:
400 ul

List Price:

$495.00

Datasheet
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