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Western blot analysis in 293 cell line lysates (35ug/lane).
B4GALNT3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in 293 cell line lysates (35ug/lane).

B4GALNT3 Antibody

CATALOG NUMBER: 57-182

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This B4GALNT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 555-584 amino acids from the Central region of human B4GALNT3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
115 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
B4GALNT3
Additional Names:
Beta-1,4-N-acetylgalactosaminyltransferase 3, B4GalNAcT3, Beta4GalNAc-T3, Beta4GalNAcT3, Beta-1,4-N-acetylgalactosaminyltransferase III, N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 2, NGalNAc-T2, B4GALNT3
Protein Accession Number:
Q6L9W6
Protein Gi Number:
161789024
Ncbi Gene Id Number:
283358
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
B4GALNT3 transfers N-acetylgalactosamine (GalNAc) ontoglucosyl residues to form N,N-prime-diacetyllactosediamine(LacdiNAc, or LDN), a unique terminal structure of cell surfaceN-glycans (Ikehara et al., 2006 [PubMed 16728562]).[supplied byOMIM].
Background Reference 1:
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 2:
Arking, D.E., et al. PLoS ONE(3), E9879 (2010) :
Background Reference 3:
Miller, E., et al. J. Biol. Chem. 283(4):1985-1991(2008)
Background Reference 4:
Huang, J., et al. Mol. Cancer Res. 5(6):543-552(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-182

List Size:
400 ul

List Price:

$495.00

Datasheet
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