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Western blot analysis in mouse kidney tissue lysates (35ug/lane).
ABHD4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse kidney tissue lysates (35ug/lane).

ABHD4 Antibody

CATALOG NUMBER: 57-192

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This ABHD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-127 amino acids from the Central region of human ABHD4.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ABHD4
Additional Names:
Abhydrolase domain-containing protein 4, 311-, Alpha/beta-hydrolase 4, Lyso-N-acylphosphatidylethanolamine lipase, ABHD4
Protein Accession Number:
Q8TB40
Protein Gi Number:
74762601
Ncbi Gene Id Number:
63874
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine (By similarity).
Background Reference 1:
Simon, G.M., et al. J. Biol. Chem. 281(36):26465-26472(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-192

List Size:
400 ul

List Price:

$495.00

Datasheet
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