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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Mouse Akt2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

Mouse Akt2 Antibody

CATALOG NUMBER: 57-345

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This Mouse Akt2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-122 amino acids from the N-terminal region of mouse Akt2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
56 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Akt2
Additional Names:
RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta, Akt2
Protein Accession Number:
Q60823
Ncbi Gene Id Number:
11652
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
General protein kinase capable of phosphorylating several known proteins.
Background Reference 1:
Zhou, F., et al. Am. J. Pathol. 177(4):2124-2133(2010) Mouton, V., et al. Biochem. J. 431(2):267-275(2010) Lazorchak, A.S., et al. Mol. Cell 39(3):433-443(2010) Goncalves, M.D., et al. PLoS ONE(9), E12707 (2010)Rotte, A., et al. Cell. Physiol. Biochem. 25(6):695-704(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-345

List Size:
400 ul

List Price:

$495.00

Datasheet
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