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Western blot analysis in mouse liver tissue lysates (35ug/lane).
Western blot analysis of Nek6 using rabbit polyclonal Mouse Nek6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Nek6 gene.
Mouse Nek6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse liver tissue lysates (35ug/lane).

Mouse Nek6 Antibody

CATALOG NUMBER: 57-356

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Pig, Rat
Immunogen:
This Mouse Nek6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-280 amino acids from the C-terminal region of mouse Nek6.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Nek6
Additional Names:
Serine/threonine-protein kinase Nek6, Never in mitosis A-related kinase 6, NimA-related protein kinase 6, Nek6
Protein Accession Number:
Q9ES70
Ncbi Gene Id Number:
59126
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis (By similarity).
Background Reference 1:
Jeon, Y.J., et al. J. Biol. Chem. 285(36):28126-28133(2010)
Background Reference 2:
Kaput, J., et al. Physiol. Genomics 18(3):316-324(2004)
Background Reference 3:
Visel, A., et al. Nucleic Acids Res. 32 (DATABASE ISSUE), D552-D556 (2004) :
Background Reference 4:
Hashimoto, Y., et al. Biochem. Biophys. Res. Commun. 293(2):753-758(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-356

List Size:
400 ul

List Price:

$495.00

Datasheet
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