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Western blot analysis in A549 cell line lysates (35ug/lane).
Fluorescent confocal image of Hela cell stained with NFYB Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFYB primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFYB immunoreactivity is localized to nucleus significantly.
Western blot analysis in A549 cell line lysates (35ug/lane).

NFYB Antibody

CATALOG NUMBER: 57-655

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat
Immunogen:
This NFYB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human NFYB.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
23 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NFYB
Additional Names:
Nuclear transcription factor Y subunit beta, CAAT box DNA-binding protein subunit B, Nuclear transcription factor Y subunit B, NF-YB, NFYB, HAP3
Protein Accession Number:
P25208
Protein Gi Number:
399193
Ncbi Gene Id Number:
4801
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is one subunit of atrimeric complex, forming a highly conserved transcription factorthat binds with high specificity to CCAAT motifs in the promoterregions in a variety of genes. This gene product, subunit B, formsa tight dimer with the C subunit, a prerequisite for subunit Aassociation. The resulting trimer binds to DNA with highspecificity and affinity. Subunits B and C each contain ahistone-like motif. Observation of the histone nature of thesesubunits is supported by two types of evidence; protein sequencealignments and experiments with mutants.
Background Reference 1:
Stros, M., et al. Nucleic Acids Res. 37(7):2070-2086(2009)
Background Reference 2:
Kang, J.H., et al. J. Cell. Biochem. 104(4):1192-1203(2008)
Background Reference 3:
Raju, C.S., et al. Mol. Biol. Cell 19(7):3008-3019(2008)
Background Reference 4:
Di Agostino, S., et al. Cancer Cell 10(3):191-202(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-655

List Size:
400 ul

List Price:

$495.00

Datasheet
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