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Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).

ASAP1 Antibody

CATALOG NUMBER: 57-774

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This ASAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-388 amino acids from the Central region of human ASAP1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
125 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ASAP1
Additional Names:
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1, 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein, ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, DEF-1, Differentiation-enhancing factor 1, PIP2-dependent ARF1 GAP, ASAP1, DDEF1, KIAA1249
Protein Accession Number:
Q9ULH1
Protein Gi Number:
296439459
Ncbi Gene Id Number:
50807
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity).
Background Reference 1:
Muller, T., et al. Oncogene 29(16):2393-2403(2010)
Background Reference 2:
Nat. Genet. 41(7):824-828(2009)
Background Reference 3:
Jian, X., et al. J. Biol. Chem. 284(3):1652-1663(2009)
Background Reference 4:
Inoue, H., et al. Mol. Biol. Cell 19(10):4224-4237(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-774

List Size:
400 ul

List Price:

$495.00

Datasheet
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