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Western blot analysis in T47D cell line lysates (35ug/lane).
Western blot analysis in T47D cell line lysates (35ug/lane).

ARFGAP3 Antibody

CATALOG NUMBER: 57-785

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ARFGAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-473 amino acids from the C-terminal region of human ARFGAP3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARFGAP3
Additional Names:
ADP-ribosylation factor GTPase-activating protein 3, ARF GAP 3, ARFGAP3, ARFGAP1
Protein Accession Number:
Q9NP61
Protein Gi Number:
21263420
Ncbi Gene Id Number:
26286
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a GTPase-activatingprotein (GAP) that associates with the Golgi apparatus andregulates the early secretory pathway of proteins. The encodedprotein promotes hydrolysis of ADP-ribosylation factor 1(ARF1)-bound GTP, which is required for the dissociation of coatproteins from Golgi-derived membranes and vesicles. Dissociation ofthe coat proteins is a prerequisite for the fusion of thesevesicles with target compartments. The activity of this protein issensitive to phospholipids. Multiple transcript variants encodingdifferent isoforms have been found for this gene. This gene wasoriginally known as ARFGAP1, but that is now the name of a relatedbut different gene.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Saitoh, A., et al. J. Biol. Chem. 284(20):13948-13957(2009)
Background Reference 3:
Weimer, C., et al. J. Cell Biol. 183(4):725-735(2008)
Background Reference 4:
Frigerio, G., et al. Traffic 8(11):1644-1655(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-785

List Size:
400 ul

List Price:

$495.00

Datasheet
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