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Western blot analysis in HL-60 cell line lysates (35ug/lane).
Western blot analysis of HAT1 using rabbit polyclonal HAT1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HAT1 gene.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

HAT1 Antibody

CATALOG NUMBER: 57-822

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This HAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human HAT1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HAT1
Additional Names:
Histone acetyltransferase type B catalytic subunit, Histone acetyltransferase 1, HAT1, KAT1
Protein Accession Number:
O14929
Ncbi Gene Id Number:
8520
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a type B histoneacetyltransferase (HAT) that is involved in the rapid acetylationof newly synthesized cytoplasmic histones, which are in turnimported into the nucleus for de novo deposition onto nascent DNAchains. Histone acetylation, particularly of histone H4, plays animportant role in replication-dependent chromatin assembly.Specifically, this HAT can acetylate soluble but not nucleosomalhistone H4 at lysines 5 and 12, and to a lesser degree, histone H2Aat lysine 5. Alternatively spliced transcript variants have beenidentified for this gene.
Background Reference 1:
Saade, E., et al. Proteomics 9(21):4934-4943(2009)
Background Reference 2:
Miyamoto, N., et al. J. Biol. Chem. 283(26):18218-18226(2008)
Background Reference 3:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Background Reference 4:
Lamesch, P., et al. Genomics 89(3):307-315(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-822

List Size:
400 ul

List Price:

$495.00

Datasheet
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