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Western blot analysis in 293 cell line lysates (35ug/lane).
MCM6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in 293 cell line lysates (35ug/lane).

MCM6 Antibody

CATALOG NUMBER: 57-953

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This MCM6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of human MCM6.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
93 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MCM6
Additional Names:
DNA replication licensing factor MCM6, p105MCM, MCM6
Protein Accession Number:
Q14566
Protein Gi Number:
2497824
Ncbi Gene Id Number:
4175
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is one of the highlyconserved mini-chromosome maintenance proteins (MCM) that areessential for the initiation of eukaryotic genome replication. Thehexameric protein complex formed by the MCM proteins is a keycomponent of the pre-replication complex (pre_RC) and may beinvolved in the formation of replication forks and in therecruitment of other DNA replication related proteins. The MCMcomplex consisting of this protein and MCM2, 4 and 7 proteinspossesses DNA helicase activity, and may act as a DNA unwindingenzyme. The phosphorylation of the complex by CDC2 kinase reducesthe helicase activity, suggesting a role in the regulation of DNAreplication.
Background Reference 1:
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Background Reference 2:
Timpson, N.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1341-1348(2010)
Background Reference 3:
Wei, Z., et al. J. Biol. Chem. 285(17):12469-12473(2010)
Background Reference 4:
Upton, J., et al. N. Z. Med. J. 123 (1308), 123 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-953

List Size:
400 ul

List Price:

$495.00

Datasheet
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