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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
CD70 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

CD70 Antibody

CATALOG NUMBER: 57-954

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CD70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 amino acids from the Central region of human CD70.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD70
Additional Names:
CD70 antigen, CD27 ligand, CD27-L, Tumor necrosis factor ligand superfamily member 7, CD70, CD70, CD27L, CD27LG, TNFSF7
Protein Accession Number:
P32970
Protein Gi Number:
21431837
Ncbi Gene Id Number:
970
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a cytokine thatbelongs to the tumor necrosis factor (TNF) ligand family. Thiscytokine is a ligand for TNFRSF27/CD27. It is a surface antigen onactivated, but not on resting, T and B lymphocytes. It inducesproliferation of costimulated T cells, enhances the generation ofcytolytic T cells, and contributes to T cell activation. Thiscytokine is also reported to play a role in regulating B-cellactivation, cytotoxic function of natural killer cells, andimmunoglobulin sythesis.
Background Reference 1:
Arimoto-Miyamoto, K., et al. Immunology 130(1):137-149(2010)
Background Reference 2:
Shaw, J., et al. Blood 115(15):3051-3057(2010)
Background Reference 3:
Yu, S.E., et al. Mol. Cells 29(2):217-221(2010)
Background Reference 4:
Kozlowska, A., et al. J. Rheumatol. 37(1):53-59(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-954

List Size:
400 ul

List Price:

$495.00

Datasheet
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