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Western blot analysis in Hela cell line lysates (35ug/lane).
Fluorescent confocal image of U251 cell stained with TADA3L Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TADA3L primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TADA3L immunoreactivity is localized to Nucleus significantly.
Western blot analysis in Hela cell line lysates (35ug/lane).

TADA3L Antibody

CATALOG NUMBER: 57-974

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Zebrafish, Mouse, Rat
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
49 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TADA3
Additional Names:
Transcriptional adapter 3, ADA3 homolog, hADA3, STAF54, Transcriptional adapter 3-like, ADA3-like protein, TADA3, ADA3, TADA3L
Protein Accession Number:
O75528
Ncbi Gene Id Number:
10474
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Many DNA-binding transcriptional activator proteinsenhance the initiation rate of RNA polymerase II-mediated genetranscription by interacting functionally with the generaltranscription machinery bound at the basal promoter. Adaptorproteins are usually required for this activation, possibly toacetylate and destabilize nucleosomes, thereby relieving chromatinconstraints at the promoter. The protein encoded by this gene is atranscriptional activator adaptor and has been found to be part ofthe PCAF histone acetylase complex. In addition, it associates withthe tumor suppressor protein p53 and is required for full activityof p53 and p53-mediated apoptosis. At least four alternativelyspliced variants have been found for this gene, but the full-lengthnature of some variants has not been determined. [provided byRefSeq].
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Li, C.W., et al. Nucleic Acids Res. 38(16):5291-5303(2010)
Background Reference 3:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Background Reference 4:
Hu, Y., et al. Cancer Invest. 27(3):298-306(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-974

List Size:
400 ul

List Price:

$495.00

Datasheet
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