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Western blot analysis of lysate from human placenta tissue lysate, using ALPI Antibody at 1:1000.
Immunohistochemical analysis of paraffin-embedded H. small intestine section using Alkaline Phosphatase (ALPI) Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysate from human placenta tissue,using ALPI Antibody at 1:1000 at each lane
Fluorescent confocal image of HeLa cells stained with Alkaline Phosphatase (ALPI) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Alkaline Phosphatase (ALPI) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Alkaline Phosphatase (ALPI) immunoreactivity is localized to the cytoplasm of HeLa cells.
Western blot analysis of lysate from human placenta tissue lysate, using ALPI Antibody at 1:1000.

Alkaline Phosphatase Antibody

CATALOG NUMBER: 58-008

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This Alkaline Phosphatase (ALPI) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the Central region of human Alkaline Phosphatase (ALPI).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:100
For IF starting dilution is: 1:100
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ALPI
Additional Names:
Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI
Protein Accession Number:
P09923
Protein Gi Number:
130744
Ncbi Gene Id Number:
248
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.
Background Reference 1:
References for protein:
Background Reference 2:
Torres,M.I., Pathol. Res. Pract. 203 (6), 485-487 (2007)
Background Reference 3:
Olsen,L., Am. J. Physiol. Gastrointest. Liver Physiol. 289 (2), G220-G226(2005)
Background Reference 4:
Alkhoury,F., Am. J. Physiol. Gastrointest. Liver Physiol. 289 (2), G285-G290(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-008

List Size:
400 ul

List Price:

$519.00

Datasheet
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