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Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human heart lysate Lane 4: mouse liver lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human heart lysate Lane 4: mouse liver lysate Lysates/proteins at 20 ug per lane.

ATF5 Antibody

CATALOG NUMBER: 58-150

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This ATF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-216 amino acids from the C-terminal region of human ATF5.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
31 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATF5
Additional Names:
Cyclic AMP-dependent transcription factor ATF-5, cAMP-dependent transcription factor ATF-5, Activating transcription factor 5, Transcription factor ATFx, ATF5, ATFX
Protein Accession Number:
Q9Y2D1
Protein Gi Number:
308153647
Ncbi Gene Id Number:
22809
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4.
Background Reference 1:
Wei, Y., et al. J. Biochem. 148(2):171-178(2010)
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Tomppo, L., et al. Biol. Psychiatry 65(12):1055-1062(2009)
Background Reference 4:
Li, G., et al. Mol. Cancer Res. 7(6):933-943(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-150

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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