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Western Blot at 1:1000 dilution Lane 1: Daudi whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
STAT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution Lane 1: Daudi whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.

STAT2 Antibody

CATALOG NUMBER: 58-173

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This STAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 820-851 amino acids from the C-terminal region of human STAT2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
98 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
STAT2
Additional Names:
Signal transducer and activator of transcription 2, p113, STAT2
Protein Accession Number:
P52630
Protein Gi Number:
1711552
Ncbi Gene Id Number:
6773
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
STAT2 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus.
Background Reference 1:
Rodriguez, J.J., et al., J. Virol. 77(21):11842-11845 (2003).
Background Reference 2:
Gotoh, B., et al., J. Virol. 77(6):3360-3370 (2003).
Background Reference 3:
Saleh, A.Z., et al., Biochemistry 41(37):11261-11268 (2002).
Background Reference 4:
Rodriguez, J.J., et al., J. Virol. 76(22):11476-11483 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-173

List Size:
400 ul

List Price:

$495.00

Datasheet
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