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Fluorescent image of A549 cell stained with TCF25 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TCF25 immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis in CEM cell line lysates (35ug/lane).
Fluorescent image of A549 cell stained with TCF25 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TCF25 immunoreactivity is localized to Cytoplasm significantly.

TCF25 Antibody

CATALOG NUMBER: 59-087

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
77 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TCF25
Additional Names:
Transcription factor 25, TCF-25, Nuclear localized protein 1, TCF25, KIAA1049, NULP1
Protein Accession Number:
Q9BQ70
Protein Gi Number:
23396596
Ncbi Gene Id Number:
22980
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TCF25 is a member of the basic helix-loop-helix (bHLH)family of transcription factors that are important in embryonicdevelopment (Steen and Lindholm, 2008 [PubMed 18068114]).[suppliedby OMIM].
Background Reference 1:
Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
Background Reference 2:
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Background Reference 3:
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Background Reference 4:
Steen, H., et al. Biochem. Biophys. Res. Commun. 366(2):432-437(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-087

List Size:
400 ul

List Price:

$495.00

Datasheet
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