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Fluorescent image of A549 cell stained with TFAP2D Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAP2D primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TFAP2D immunoreactivity is localized to Nucleus significantly.
Western blot analysis in HL-60 cell line lysates (35ug/lane).
Fluorescent image of A549 cell stained with TFAP2D Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAP2D primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TFAP2D immunoreactivity is localized to Nucleus significantly.

TFAP2D Antibody

CATALOG NUMBER: 59-530

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TFAP2D
Additional Names:
Transcription factor AP-2-delta, AP2-delta, Activating enhancer-binding protein 2-delta, Transcription factor AP-2-beta-like 1, TFAP2D (HGNC:15581)
Protein Accession Number:
Q7Z6R9
Protein Gi Number:
74750179
Ncbi Gene Id Number:
83741
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC (By similarity).
Background Reference 1:
Kim, J.M., et al. DNA Res. 13(6):275-286(2006)
Background Reference 2:
Wenke, A.K., et al. Biochem. Biophys. Res. Commun. 345(1):495-501(2006)
Background Reference 3:
Cheng, C., et al. Int. J. Biochem. Cell Biol. 34(1):78-86(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-530

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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