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Western blot analysis in CEM cell line lysates (35ug/lane).
Western blot analysis in CEM cell line lysates (35ug/lane).

DPH2 Antibody

CATALOG NUMBER: 59-759

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-148 amino acids from the N-terminal region of human DPH2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DPH2
Additional Names:
Diphthamide biosynthesis protein 2, DPH2 homolog, HsDph2, Diphthamide biosynthesis protein 2 homolog-like 2, DPH2-like 2, DPH2, DPH2L2
Protein Accession Number:
Q9BQC3
Protein Gi Number:
74761201
Ncbi Gene Id Number:
1802
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is one of two human genes similar to the yeastgene dph2. The yeast gene was identified by its ability tocomplement a diphthamide mutant strain, and thus probably functionsin diphthamide biosynthesis. Diphthamide is a post-translationallymodified histidine residue present in elongation factor 2 (EF2)that is the target of diphtheria toxin ADP-ribosylation. Twotranscript variants encoding different isoforms have been found forthis gene.
Background Reference 1:
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 2:
Liu, S., et al. Mol. Cell. Biol. 24(21):9487-9497(2004)
Background Reference 3:
Schultz, D.C., et al. Genomics 52(2):186-191(1998)
Background Reference 4:
Foley, B.T., et al. J. Biol. Chem. 270(39):23218-23225(1995)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-759

List Size:
400 ul

List Price:

$495.00

Datasheet
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