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Western blot analysis in CEM cell line lysates (35ug/lane).
Western blot analysis in mouse liver tissue lysates (35ug/lane).
Western blot analysis in CEM cell line lysates (35ug/lane).

CCNF Antibody

CATALOG NUMBER: 59-800

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CCNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496-524 amino acids from the Central region of human CCNF.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
88 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CCNF
Additional Names:
Cyclin-F, F-box only protein 1, CCNF, FBX1, FBXO1
Protein Accession Number:
P41002
Protein Gi Number:
20178283
Ncbi Gene Id Number:
899
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the cyclin family. Cyclinsare important regulators of cell cycle transitions through theirability to bind and activate cyclin-dependent protein kinases. Thismember also belongs to the F-box protein family which ischaracterized by an approximately 40 amino acid motif, the F-box.The F-box proteins constitute one of the four subunits of theubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box),which function in phosphorylation-dependent ubiquitination. TheF-box proteins are divided into 3 classes: Fbws containing WD-40domains, Fbls containing leucine-rich repeats, and Fbxs containingeither different protein-protein interaction modules or norecognizable motifs. The protein encoded by this gene belongs tothe Fbxs class and it was one of the first proteins in which theF-box motif was identified.
Background Reference 1:
D'Angiolella, V., et al. Nature 466(7302):138-142(2010)
Background Reference 2:
Fung, T.K., et al. J. Biol. Chem. 277(38):35140-35149(2002)
Background Reference 3:
Kong, M., et al. EMBO J. 19(6):1378-1388(2000)
Background Reference 4:
Bai, C., et al. Cell 86(2):263-274(1996)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-800

List Size:
400 ul

List Price:

$495.00

Datasheet
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