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Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).

B4GALNT1 Antibody

CATALOG NUMBER: 59-823

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This B4GALNT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-323 amino acids from the Central region of human B4GALNT1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
B4GALNT1
Additional Names:
Beta-1,4 N-acetylgalactosaminyltransferase 1, (N-acetylneuraminyl)-galactosylglucosylceramide, GM2/GD2 synthase, GalNAc-T, B4GALNT1, GALGT, SIAT2
Protein Accession Number:
Q00973
Protein Gi Number:
1168736
Ncbi Gene Id Number:
2583
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GM2 and GD2 gangliosides are sialic acid-containingglycosphingolipids. GalNAc-T is the enzyme involved in thebiosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-Tcatalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4linkage, resulting in the synthesis of G(M2) and G(D2),respectively.
Background Reference 1:
Chen, L.C., et al. Lung Cancer 67(2):216-220(2010)
Background Reference 2:
Boraska, V., et al. Clin. Biochem. 42(9):819-822(2009)
Background Reference 3:
Gornati, R., et al. Mol. Cell. Biochem. 298 (1-2), 59-68 (2007) :
Background Reference 4:
Li, J., et al. J. Biol. Chem. 275(52):41476-41486(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-823

List Size:
400 ul

List Price:

$495.00

Datasheet
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