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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

PODXL Antibody

CATALOG NUMBER: 60-255

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PODXL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the C-terminal region of human PODXL.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PODXL
Additional Names:
Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1, PC, PCLP-1, PODXL, PCLP, PCLP1
Protein Accession Number:
O00592
Ncbi Gene Id Number:
5420
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the sialomucin proteinfamily. The encoded protein was originally identified as animportant component of glomerular podocytes. Podocytes are highlydifferentiated epithelial cells with interdigitating foot processescovering the outer aspect of the glomerular basement membrane.Other biological activities of the encoded protein include: bindingin a membrane protein complex with Na+/H+ exchanger regulatoryfactor to intracellular cytoskeletal elements, playing a role inhematopoetic cell differentiation, and being expressed in vascularendothelium cells and binding to L-selectin.
Background Reference 1:
Schopperle, W.M., et al. Biochem. Biophys. Res. Commun. 398(3):372-376(2010)
Background Reference 2:
Hsu, Y.H., et al. Am. J. Pathol. 176(6):3050-3061(2010)
Background Reference 3:
Johnatty, S.E., et al. PLoS Genet.(7), E1001016 (2010) :
Background Reference 4:
Drossopoulou, G.I., et al. Am. J. Physiol. Renal Physiol. 297 (3), F594-F603 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-255

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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