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Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.

SRP14 Antibody

CATALOG NUMBER: 60-309

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This SRP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-55 amino acids from the Central region of human SRP14.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
15 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SRP14
Additional Names:
Signal recognition particle 14 kDa protein, SRP14, 18 kDa Alu RNA-binding protein, SRP14
Protein Accession Number:
P37108
Protein Gi Number:
116242801
Ncbi Gene Id Number:
6727
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.
Background Reference 1:
Mary, C., et al. RNA 16(5):969-979(2010)
Background Reference 2:
Lakkaraju, A.K., et al. Cell 133(3):440-451(2008)
Background Reference 3:
Yoshida, M., et al. Mol. Pharmacol. 73(3):987-994(2008)
Background Reference 4:
Lamesch, P., et al. Genomics 89(3):307-315(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-309

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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