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Western blot analysis in HL-60 cell line lysates (35ug/lane).
Western blot analysis in HL-60 cell line lysates (35ug/lane).

RLIM Antibody

CATALOG NUMBER: 60-843

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RLIM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-227 amino acids from the Central region of human RLIM.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
69 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RLIM
Additional Names:
E3 ubiquitin-protein ligase RLIM, 632-, LIM domain-interacting RING finger protein, RING finger LIM domain-binding protein, R-LIM, RING finger protein 12, Renal carcinoma antigen NY-REN-43, RLIM, RNF12
Protein Accession Number:
Q9NVW2
Protein Gi Number:
143811451
Ncbi Gene Id Number:
51132
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a RING-H2 zinc fingerprotein. It has been shown to be an E3 ubiquitin protein ligasethat targets LIM domain binding 1 (LDB1/CLIM), and causesproteasome-dependent degradation of LDB1. This protein and LDB1 areco-repressors of LHX1/LIM-1, a homeodomain transcription factor.Multiple alternatively spliced variants, encoding the same protein,have been identified.
Background Reference 1:
Jonkers, I., et al. Cell 139(5):999-1011(2009)
Background Reference 2:
Her, Y.R., et al. J. Biol. Chem. 284(13):8557-8566(2009)
Background Reference 3:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Background Reference 4:
Hiratani, I., et al. Development 130(17):4161-4175(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-843

List Size:
400 ul

List Price:

$495.00

Datasheet
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