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Western blot analysis in A549 cell line lysates (35ug/lane).
Western blot analysis in A549 cell line lysates (35ug/lane).

HIF1A Antibody

CATALOG NUMBER: 60-903

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This HIF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 735-762 amino acids from the C-terminal region of human HIF1A.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
93 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HIF1A
Additional Names:
Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8
Protein Accession Number:
Q16665
Protein Gi Number:
2498017
Ncbi Gene Id Number:
3091
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Hypoxia-inducible factor-1 (HIF1) is a transcriptionfactor found in mammalian cells cultured under reduced oxygentension that plays an essential role in cellular and systemichomeostatic responses to hypoxia. HIF1 is a heterodimer composed ofan alpha subunit and a beta subunit. The beta subunit has beenidentified as the aryl hydrocarbon receptor nuclear translocator(ARNT). This gene encodes the alpha subunit of HIF-1.Overexpression of a natural antisense transcript (aHIF) of thisgene has been shown to be associated with nonpapillary renalcarcinomas. Two alternative transcripts encoding different isoformshave been identified.
Background Reference 1:
Gonsalves, C., et al. J. Immunol. 185(10):6253-6264(2010)
Background Reference 2:
Hindryckx, P., et al. J. Immunol. 185(10):6306-6316(2010)
Background Reference 3:
Espinosa, I., et al. Am. J. Surg. Pathol. 34(11):1708-1714(2010)
Background Reference 4:
Corzo, C.A., et al. J. Exp. Med. 207(11):2439-2453(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-903

List Size:
400 ul

List Price:

$495.00

Datasheet
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