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Fluorescent image of HepG2 cells stained with AK4 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded H.kidney section using AK4 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M.kidney section using AK4 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H.heart section using AK4 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from A431, Hela, HepG2, MCF-7, Raji cell line and mouse kidney tissue lysate (from left to right), using AK4 Antibody at 1:1000 at each lane.
Fluorescent image of HepG2 cells stained with AK4 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

AK4 Antibody

CATALOG NUMBER: 61-029

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This AK4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 119-153 amino acids from the Central region of human AK4.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IF starting dilution is: 1:25
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
25 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AK4
Additional Names:
Adenylate kinase 4, mitochondrial {ECO:0000255, HAMAP-Rule:MF_03170}, AK 4 {ECO:0000255, HAMAP-Rule:MF_03170}, 27410 {ECO:0000255, HAMAP-Rule:MF_03170}, 2746 {ECO:0000255, HAMAP-Rule:MF_03170}, Adenylate kinase 3-like {ECO:0000255, HAMAP-Rule:MF_03170}, GTP:AMP phosphotransferase AK4 {ECO:0000255, HAMAP-Rule:MF_03170}, AK4 {ECO:0000255, HAMAP-Rule:MF_03170}
Protein Accession Number:
P27144
Protein Gi Number:
125157
Ncbi Gene Id Number:
1.00508E+11
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity.
Background Reference 1:
Xu G.,et al.Genomics 13:537-542(1992).
Background Reference 2:
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Background Reference 3:
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Background Reference 4:
Gregory S.G.,et al.Nature 441:315-321(2006).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-029

List Size:
400 ul

List Price:

$519.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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