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Western blot analysis of E1 Ubiquitin (UBE1) Antibody in mouse stomach tissue lysates (35ug/lane)
Antibody is used in Western blot to detect UBE1 in HL-60 cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with UBE1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of E1 Ubiquitin (UBE1) Antibody in mouse stomach tissue lysates (35ug/lane)

E1 Ubiquitin Antibody

CATALOG NUMBER: 61-077

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rabbit, Rat
Immunogen:
This E1 Ubiquitin (UBE1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1026-1058 amino acids from the C-terminal region of human E1 Ubiquitin (UBE1).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
118 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBA1
Additional Names:
Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1, UBA1, A1S9T, UBE1
Protein Accession Number:
P22314
Protein Gi Number:
24418865
Ncbi Gene Id Number:
7317
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR.
Background Reference 1:
Ayusawa, D., et al., Cell Struct. Funct. 17(2):113-122 (1992). Handley, P.M., et al., Proc. Natl. Acad. Sci. U.S.A. 88(1):258-262 (1991). Kudo, M., et al., Exp. Cell Res. 192(1):110-117 (1991). Zacksenhaus, E., et al., Cytogenet. Cell Genet. 53(1):20-22 (1990). Zacksenhaus, E., et al., EMBO J. 9(9):2923-2929 (1990).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-077

List Size:
400 ul

List Price:

$495.00

Datasheet
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