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Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
ARHGAP25 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

ARHGAP25 Antibody

CATALOG NUMBER: 61-689

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ARHGAP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-219 amino acids from the Central region of human ARHGAP25.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
73 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit IgG
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARHGAP25
Additional Names:
Rho GTPase-activating protein 25, Rho-type GTPase-activating protein 25, ARHGAP25, KIAA0053
Protein Accession Number:
P42331
Protein Gi Number:
238054314
Ncbi Gene Id Number:
9938
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ARHGAP25 encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration.
Background Reference 1:
Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010)
Background Reference 2:
Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009)
Background Reference 3:
Katoh, M., et al. Int. J. Mol. Med. 14(2):333-338(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-689

List Size:
400 ul

List Price:

$495.00

Datasheet
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