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Western blot analysis of CD80 Antibody in HL-60 cell line lysates (35ug/lane)
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CD80 Antibody in HL-60 cell line lysates (35ug/lane)

CD80 Antibody

CATALOG NUMBER: 61-757

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CD80 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-288 amino acids from the C-terminal region of human CD80.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD80
Additional Names:
T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B71, B7, CD80, CD80, CD28LG, CD28LG1, LAB7
Protein Accession Number:
P33681
Protein Gi Number:
461606
Ncbi Gene Id Number:
941
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD80 is activation antigen B7-1 (formerly referred to as B7) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 (MIM 186760) and CTLA4 (MIM 123890) ligands of T cells.
Background Reference 1:
Mosbruger, T.L., et al. J. Infect. Dis. 201(9):1371-1380(2010)
Background Reference 2:
Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
Background Reference 3:
Segat, L., et al. J. Gastroenterol. Hepatol. 24(12):1840-1846(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-757

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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