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Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstratesdetected the CCND1 protein (arrow).
Confocal immunofluorescent analysis of CCND1-Y226 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstratesdetected the CCND1 protein (arrow).

CCND1-Y226 Antibody

CATALOG NUMBER: 61-878

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CCND1
Additional Names:
G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1
Protein Accession Number:
P24385
Protein Gi Number:
116152
Ncbi Gene Id Number:
595
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.
Background Reference 1:
Edel, M.J., et al. Genes Dev. 24(6):561-573(2010)
Background Reference 2:
Zhong, Z., et al. Cancer Res. 70(5):2105-2114(2010)
Background Reference 3:
Kanaan, Z., et al. Int. J. Biol. Markers 25(1):27-31(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-878

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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