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Western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the detected B4GALT5 protein (arrow).
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the detected B4GALT5 protein (arrow).

B4GALT5 Antibody

CATALOG NUMBER: 61-901

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This B4GALT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 324-350 amino acids from the C-terminal region of human B4GALT5.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
B4GALT5
Additional Names:
Beta-1,4-galactosyltransferase 5, Beta-1,4-GalTase 5, Beta4Gal-T5, b4Gal-T5, 241-, Beta-1,4-GalT II, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5, B4GALT5
Protein Accession Number:
O43286
Ncbi Gene Id Number:
9334
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is one of seven beta-1,4-galactosyltransferase(beta4GalT) genes. They encode type II membrane-bound glycoproteinsthat appear to have exclusive specificity for the donor substrateUDP-galactose; all transfer galactose in a beta1,4 linkage tosimilar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has adistinct function in the biosynthesis of different glycoconjugatesand saccharide structures. As type II membrane proteins, they havean N-terminal hydrophobic signal sequence that directs the proteinto the Golgi apparatus and which then remains uncleaved to functionas a transmembrane anchor. By sequence similarity, the beta4GalTsform four groups: beta4GalT1 and beta4GalT2, beta4GalT3 andbeta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The functionof the enzyme encoded by this gene is not clear. This gene waspreviously designated as B4GALT4 but was renamed to B4GALT5. In theliterature it is also referred to as beta4GalT2. [provided byRefSeq].
Background Reference 1:
Kitayama, K., et al. J. Biol. Chem. 282(41):30085-30096(2007)
Background Reference 2:
Sato, T., et al. J. Biol. Chem. 282(38):27702-27712(2007)
Background Reference 3:
Jiang, J., et al. J. Biol. Chem. 281(14):9482-9489(2006)
Background Reference 4:
Sato, T., et al. J. Biol. Chem. 279(38):39574-39583(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-901

List Size:
400 ul

List Price:

$495.00

Datasheet
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