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Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

GLP1 Antibody

CATALOG NUMBER: 61-954

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This GLP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-112 amino acids from the Central region of human GLP1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
30 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZGLP1
Additional Names:
GATA-type zinc finger protein 1, GATA-like protein 1, GLP-1, ZGLP1, GLP1
Protein Accession Number:
P0C6A0
Protein Gi Number:
166990464
Ncbi Gene Id Number:
100125288
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Glucagon is initially synthesised as proglucagon, which is 180 amino acids in length and is cleaved proteolytically. It contains a region corresponding to Glucagon itself, in addition to a signal peptide, spacer regions and two Glucagon-like peptide sequences. GLP1 is encoded by the fourth exon of the six in the human Glucagon gene. It is 37 amino acids in length and has 48% amino acid sequence homology to Glucagon, and is thought to have arisen by gene duplication. The sequence of GLP1 is conserved across many species, however, indicating that it has a particular function. GLP1 is a potent insulin secretagogue, renders pancreatic beta cells 'glucose-competent' and may be useful in the treatment of diabetes mellitus.
Background Reference 1:
Sanz, C., et al. Am. J. Physiol. Endocrinol. Metab. 298 (3), E634-E643 (2010) :
Background Reference 2:
Brown, R.J., et al. Diabetes Care 32(12):2184-2186(2009)
Background Reference 3:
Li, S., et al. Dev. Biol. 301(1):106-116(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-954

List Size:
400 ul

List Price:

$495.00

Datasheet
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