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Western blot analysis in T47D cell line lysates (35ug/lane).
Western blot analysis of ADH1C using rabbit polyclonal ADH1C Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH1C gene.
ADH1C Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of ADH1C Antibody with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in T47D cell line lysates (35ug/lane).

ADH1C Antibody

CATALOG NUMBER: 61-966

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ADH1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-260 amino acids from the Central region of human ADH1C.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
40 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ADH1C
Additional Names:
Alcohol dehydrogenase 1C, Alcohol dehydrogenase subunit gamma, ADH1C, ADH3
Protein Accession Number:
P00326
Protein Gi Number:
113398
Ncbi Gene Id Number:
126
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes class I alcohol dehydrogenase, gammasubunit, which is a member of the alcohol dehydrogenase family.Members of this enzyme family metabolize a wide variety ofsubstrates, including ethanol, retinol, other aliphatic alcohols,hydroxysteroids, and lipid peroxidation products. Class I alcoholdehydrogenase, consisting of several homo- and heterodimers ofalpha, beta, and gamma subunits, exhibits high activity for ethanoloxidation and plays a major role in ethanol catabolism. Three genesencoding alpha, beta and gamma subunits are tandemly organized in agenomic segment as a gene cluster.
Background Reference 1:
Benzon Larsen, S., et al. Cancer Lett. (2010) In press :
Background Reference 2:
Sangrajrang, S., et al. Breast Cancer Res. Treat. (2010)
Background Reference 3:
Khan, A.J., et al. Drug Alcohol Depend (2010) In press :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-966

List Size:
400 ul

List Price:

$495.00

Datasheet
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