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Western Blot at 1:1000 dilution + ZR-75-1 whole cell lysate Lysates/proteins at 20 ug per lane.
AVP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of AVP Antibody with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Western Blot at 1:1000 dilution + ZR-75-1 whole cell lysate Lysates/proteins at 20 ug per lane.

AVP Antibody

CATALOG NUMBER: 61-973

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AVP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-129 amino acids from the Central region of human AVP.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
17 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AVP
Additional Names:
Vasopressin-neurophysin 2-copeptin, AVP-NPII, Arg-vasopressin, Arginine-vasopressin, Neurophysin 2, Neurophysin-II, Copeptin, AVP, ARVP, VP
Protein Accession Number:
P01185
Protein Gi Number:
128083
Ncbi Gene Id Number:
551
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a precursor protein consisting ofarginine vasopressin and two associated proteins, neurophysin 2 anda glycopeptide, copeptin. Arginine vasopressin is a posteriorpituitary hormone which is synthesized in the supraoptic nucleusand paraventricular nucleus of the hypothalamus. Along with itscarrier protein, neurophysin 2, it is packaged into neurosecretoryvesicles and transported axonally to the nerve endings in theneurohypophysis where it is either stored or secreted into thebloodstream. The precursor is thought to be activated while it isbeing transported along the axon to the posterior pituitary.Arginine vasopressin acts as a growth factor by enhancing pHregulation through acid-base transport systems. It has a directantidiuretic action on the kidney, and also causes vasoconstrictionof the peripheral vessels. This hormone can contract smooth muscleduring parturition and lactation. It is also involved in cognition,tolerance, adaptation and complex sexual and maternal behaviour, aswell as in the regulation of water excretion and cardiovascularfunctions.
Background Reference 1:
Abu Libdeh, A., et al. Eur. J. Endocrinol. 162(2):221-226(2010)
Background Reference 2:
Cirillo, M. Kidney Int. 77(1):5-6(2010)
Background Reference 3:
Meijer, E., et al. Kidney Int. 77(1):29-36(2010)
Background Reference 4:
Birk, J., et al. J. Cell. Sci. 122 (PT 21), 3994-4002 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-973

List Size:
400 ul

List Price:

$495.00

Datasheet
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