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Western blot analysis of lysate from MCF-7, A431 cell line, using CALCR Antibody with(+) or without(-) peptides at 1:1000 at each lane. A goat anti-rabbit(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of CALCR Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of lysate from MCF-7, A431 cell line, using CALCR Antibody with(+) or without(-) peptides at 1:1000 at each lane. A goat anti-rabbit(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

CALCR Antibody

CATALOG NUMBER: 62-008

Clonality:
Polyclonal
Tested Applications:
Flow, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CALCR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from the C-terminal region of human CALCR.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CALCR
Additional Names:
Calcitonin receptor, CT-R, CALCR
Protein Accession Number:
P30988
Protein Gi Number:
550544247
Ncbi Gene Id Number:
799
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CALCR is a high affinity receptor for the peptide hormone calcitonin and belongs to a subfamily of seven transmembrane-spanning G protein-coupled receptors. The encoded protein is involved in maintaining calcium homeostasis and in regulating osteoclast-mediated bone resorption. Polymorphisms in this gene have been associated with variations in bone mineral density and onset of osteoporosis.
Background Reference 1:
Nussenzveig, D.R., et al. Endocrinology 136(5):2047-2051(1995) Nakamura, M., et al. Biochem. Biophys. Res. Commun. 209(2):744-751(1995) Egerton, M., et al. J. Mol. Endocrinol. 14(2):179-189(1995) Gorn, A.H., et al. J. Clin. Invest. 90(5):1726-1735(1992)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-008

List Size:
400 ul

List Price:

$495.00

Datasheet
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