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Western Blot at 1:1000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western Blot at 1:1000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.

NRG2 Antibody

CATALOG NUMBER: 62-186

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This NRG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-389 amino acids from the C-terminal region of human NRG2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
92 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NRG2
Additional Names:
Pro-neuregulin-2, membrane-bound isoform, Pro-NRG2, Neuregulin-2, NRG-2, Divergent of neuregulin-1, DON-1, Neural- and thymus-derived activator for ERBB kinases, NTAK, NRG2, NTAK
Protein Accession Number:
O14511
Ncbi Gene Id Number:
9542
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Neuregulin 2 (NRG2) is a novel member of the neuregulin family of growth and differentiation factors. Through interaction with the Erbb family of receptors, NRG2 induces the growth and differentiation of epithelial, neuronal, glial, and other types of cells. The gene consists of 12 exons and the genomic structure is similar to that of neuregulin 1 (NRG1), another member of the neuregulin family of ligands. NRG1 and NRG2 mediate distinct biological processes by acting at different sites in tissues and eliciting different biological responses in cells. There are six NRG2 alternatively spliced transcripts which are variable between the epidermal growth factor-like (EGF-like) and the transmembrane domains. The gene is located close to the region for demyelinating Charcot-Marie-Tooth disease locus, but is not responsible for this disease.
Background Reference 1:
Yamada, K., et al., Gene 255(1):15-24 (2000). Ring, H.Z., et al., Hum. Genet. 104(4):326-332 (1999). Busfield, S.J., et al., Mol. Cell. Biol. 17(7):4007-4014 (1997). Higashiyama, S., et al., J. Biochem. 122(3):675-680 (1997). Carraway, K.L. III, et al., Nature 387(6632):512-516 (1997).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-186

List Size:
400 ul

List Price:

$495.00

Datasheet
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