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Western blot analysis of Cdc6 Antibody in Romas cell line lysates (35ug/lane)
Western blot analysis of Cdc6 Antibody in Romas cell line lysates (35ug/lane)

Cdc6 Antibody

CATALOG NUMBER: 62-209

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This Cdc6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human Cdc6.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
63 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDC6
Additional Names:
Cell division control protein 6 homolog, CDC6-related protein, Cdc18-related protein, HsCdc18, p62(cdc6), HsCDC6, CDC6, CDC18L
Protein Accession Number:
Q99741
Protein Gi Number:
50400620
Ncbi Gene Id Number:
990
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Human Cdc6 is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.
Background Reference 1:
Alexandrow, M.G., et al., Mol. Cell. Biol. 24(4):1614-1627 (2004).
Background Reference 2:
Yim, H., et al., Mol. Biol. Cell 14(10):4250-4259 (2003).
Background Reference 3:
Clay-Farrace, L., et al., EMBO J. 22(3):704-712 (2003).
Background Reference 4:
Pelizon, C., et al., EMBO Rep. 3(8):780-784 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-209

List Size:
400 ul

List Price:

$495.00

Datasheet
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