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Western blot analysis of anti-HDGF Pab in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HDGF antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of HDGF Antibody with SK-BR-3 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Western blot analysis of anti-HDGF Pab in Hela cell line lysates (35ug/lane)

HDGF Antibody

CATALOG NUMBER: 62-269

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HDGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-197 amino acids from the C-terminal region of human HDGF.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
27 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HDGF
Additional Names:
Hepatoma-derived growth factor, HDGF, High mobility group protein 1-like 2, HMG-1L2, HDGF, HMG1L2
Protein Accession Number:
P51858
Protein Gi Number:
1708157
Ncbi Gene Id Number:
3068
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HDGF is a member of the hepatoma-derived growth factor family. This protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation.
Background Reference 1:
Thakar,K., FEBS J. 275 (7), 1411-1426 (2008)
Background Reference 2:
Chang,K.C.,Int. J. Cancer 121 (5), 1059-1065 (2007)
Background Reference 3:
Yamamoto,S., Ann. Surg. Oncol. 14 (7), 2141-2149 (2007)
Background Reference 4:
Uyama,H., Clin. Cancer Res. 12 (20 PT 1), 6043-6048 (2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-269

List Size:
400 ul

List Price:

$495.00

Datasheet
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