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Western blot analysis of ATP7B antibody in HepG2 cell line lysates (35ug/lane)
ATP7B Antibody IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of ATP7B Antibody with HepG2 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ATP7B antibody in HepG2 cell line lysates (35ug/lane)

ATP7B Antibody

CATALOG NUMBER: 62-284

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This ATP7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1361-1391 amino acids from the C-terminal region of human ATP7B.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
157 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATP7B
Additional Names:
Copper-transporting ATPase 2, Copper pump 2, Wilson disease-associated protein, WND/140 kDa, ATP7B, PWD, WC1, WND
Protein Accession Number:
P35670
Protein Gi Number:
239938919
Ncbi Gene Id Number:
540
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATP7B is a member of the P-type cation transport ATPase family and a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile.
Background Reference 1:
Martinez-Balibrea,E., Int. J. Cancer 124 (12), 2905-2910 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-284

List Size:
400 ul

List Price:

$495.00

Datasheet
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