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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ARPC1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Immunofluorescence analysis of ARPC1A Antibody with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Flow cytometric analysis of WiDr cells using ARPC1A Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

ARPC1A Antibody

CATALOG NUMBER: 62-296

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This ARPC1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human ARPC1A.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARPC1A
Additional Names:
Actin-related protein 2/3 complex subunit 1A, SOP2-like protein, ARPC1A, SOP2L
Protein Accession Number:
Q92747
Protein Gi Number:
88984001
Ncbi Gene Id Number:
10552
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ARPC1A is one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages.
Background Reference 1:
Laurila,E., Genes Chromosomes Cancer 48 (4), 330-339 (2009)
Background Reference 2:
Machesky,L.M., Biochem. J. 328 (PT 1), 105-112 (1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-296

List Size:
400 ul

List Price:

$495.00

Datasheet
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