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Western blot analysis of ACTG1 Antibody in K562, HepG2 cell line lysates (35ug/lane)
Flow cytometric analysis of K562 cells using ACTG1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ACTG1 using rabbit polyclonal ACTG1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTG1 gene.
Confocal immunofluorescent analysis of ACTG1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of ACTG1 Antibody in K562, HepG2 cell line lysates (35ug/lane)

ACTG1 Antibody

CATALOG NUMBER: 62-306

Clonality:
Polyclonal
Tested Applications:
Flow, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: C. Elegans, Drosophila, Xenopus, Chicken, Bovine, Mouse, Rabbit, Rat, Zebrafish, Hamster, Monkey, Pig, Yeast
Immunogen:
This ACTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-215 amino acids from the Central region of human ACTG1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACTG1
Additional Names:
Actin, cytoplasmic 2, Gamma-actin, Actin, cytoplasmic 2, N-terminally processed, ACTG1, ACTG
Protein Accession Number:
P63261
Protein Gi Number:
54036678
Ncbi Gene Id Number:
71
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, is a cytoplasmic actin found in nonmuscle cells.
Background Reference 1:
de Heer,A.M., Ann. Otol. Rhinol. Laryngol. 118 (5), 382-390 (2009)
Background Reference 2:
Mouilleron,S., EMBO J. 27 (23), 3198-3208 (2008)
Background Reference 3:
Liu,P.,Genet Genomics 35 (9), 553-558 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-306

List Size:
400 ul

List Price:

$495.00

Datasheet
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