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Western blot analysis of EEF1A1/ EEF1A2 Antibody in Y79, T47D cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human brain with EEF1A1/ EEF1A2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of NCI-H292 cells using EEF1A1/ EEF1A2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
With 293T cell line lysate,resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody EEF1A1/ EEF1A2 (1:500, 4°Covernight ) and horseradish peroxidase–conjugated second antibo dy(rabbit). After washing, the bound antibody complex was detected using an ECL chemiluminescence reagent and XAR film (Kodak).
Western blot analysis of EEF1A1/ EEF1A2 Antibody in Y79, T47D cell line lysates (35ug/lane).

EEF1A1/ EEF1A2 Antibody

CATALOG NUMBER: 62-351

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Rabbit, Zebrafish, Hamster, Chicken, Yeast, Xenopus, Bovine, C. Elegans, Drosophila
Immunogen:
This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids of human EEF1A1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EEF1A1
Additional Names:
Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, Leukocyte receptor cluster member 7, EEF1A1, EEF1A, EF1A, LENG7
Protein Accession Number:
P68104
Protein Gi Number:
55584035
Ncbi Gene Id Number:
1915
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.
Background Reference 1:
Byun,H.O., Cancer Res. 69 (11), 4638-4647 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-351

List Size:
400 ul

List Price:

$495.00

Datasheet
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