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Western blot analysis of CAMKK2 using rabbit polyclonal CAMKK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMKK2 gene (Lane 2).
Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMKK2 antibody (Cat.#AP711b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of CAMKK2 using rabbit polyclonal CAMKK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMKK2 gene (Lane 2).

CAMKK2 Antibody

CATALOG NUMBER: 62-671

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This CAMKK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 483-512 amino acids from the C-terminal region of human CAMKK2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50
Predicted Molecular Weight:
65 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CAMKK2
Additional Names:
Calcium/calmodulin-dependent protein kinase kinase 2, CaM-KK 2, CaM-kinase kinase 2, CaMKK 2, Calcium/calmodulin-dependent protein kinase kinase beta, CaM-KK beta, CaM-kinase kinase beta, CaMKK beta, CAMKK2, CAMKKB, KIAA0787
Protein Accession Number:
Q96RR4
Protein Gi Number:
317373374
Ncbi Gene Id Number:
10645
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CAMKK2 belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. CAMKK2 appears to be involved in hippocampal activation of CREB1.
Background Reference 1:
Hsu, L.S., et al., J. Biol. Chem. 276(33):31113-31123 (2001).
Background Reference 2:
Hsu, L.S., et al., J. Biomed. Sci. 5(2):141-149 (1998).
Background Reference 3:
Anderson, K.A., et al., J. Biol. Chem. 273(48):31880-31889 (1998).
Background Reference 4:
Ishikawa, Y., et al., FEBS Lett. 550 (1-3), 57-63 (2000).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-671

List Size:
400 ul

List Price:

$495.00

Datasheet
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