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Western blot analysis of anti-PHKG1 Pab in A375 cell lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-PHKG1 Pab in A375 cell lysate

PHKG1 Antibody

CATALOG NUMBER: 62-744

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit, Rat
Immunogen:
This PHKG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-332 amino acids from the Central region of human PHKG1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PHKG1
Additional Names:
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform, PHK-gamma-M, Phosphorylase kinase subunit gamma-1, Serine/threonine-protein kinase PHKG1, PHKG1, PHKG
Protein Accession Number:
Q16816
Protein Gi Number:
2833281
Ncbi Gene Id Number:
5260
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12.
Background Reference 1:
Wehner, M., et al., Hum. Genet. 96(5):616-618 (1995).
Background Reference 2:
Jones, T.A., et al., Biochim. Biophys. Acta 1048(1):24-29 (1990).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-744

List Size:
400 ul

List Price:

$495.00

Datasheet
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